You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1088550 |
---|---|
Category | Proteins |
Description | Recombinant Human Chitinase 3 Like 1(CHI3L1) protein is produced by Mammalian expression system and the target gene encoding Tyr22-Thr383 is expressed with a His tag at the C-terminus. The CHI3L1 protein consisting of 377 amino acids and has a calculated molecular mass of 41.47 kDa and migrates as an 46.6 kDa band in SDS-PAGE under reducing conditions due to glycosylation. Accession Number:P36222 Also known as: CHI3L1、YKL-40 |
Tested applications | CLIA, EIA, LF |
Tag | His-tag |
Concentration | Lot specific(±20%) |
Form/Appearance | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 |
Purity | > 95% as determined by SDS-PAGE. |
MW | 42.2 kDa |
Target | CHI3L1 |
UniProt ID | P36222 |
Source | HEK293 |
Expression System | HEK293 Cells |
Storage | Short term (up to 7 days) store at 2–8°C. Long term, store at -20°C. Avoid multiple freeze/thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4. |
Note | For research use only |
Application notes | Use: Calibration/quality control. Quality control: Mol Mass: 41.47 kDa AP Mol Mass: 46.6 kDa Purity: ≧90% as determined by reducing SDS-PAGE |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
60.5 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide. The apparent molecular mass of CHI3L1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
Filter by Rating