Recombinant Human CDCP1 Protein

SKU: orb2992961

Description

Recombinant Human CUB domain-containing protein 1 is produced by our Mammalian expression system and the target gene encoding Phe30-Thr667 is expressed with a 6His tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-CDCP1-NTF antibody(10D7) at 2μg/ml (100 μl/well) can bind Recombinant Human CDCP1 (C-6His)(Cat#orb2992961). The ED50 of Recombinant Human CDCP1 (C-6His)(Cat#orb2992961) is 13.06 ng/ml. (Regularly tested). BLI: Loaded Recombinant Human CDCP1 (C-6His)(Cat#orb2992961) on HIS1K Biosensor, can bind Anti-CDCP1-NTF antibody(10D7) with an affinity constant of 0.504 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 72.6 KDa. Observed: 86-125 KDa, reducing conditions
• Expression Region: Phe30-Thr667
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CUB domain-containing protein 1; CDCP1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein (SIMA135); CD318; TRASK

Quick Database Links

UniProt

Q9H5V8