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| Catalog Number | orb2995007 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human CD98 is produced by our Mammalian expression system and the target gene encoding Arg206-Ala630 is expressed with a 6His tag at the C-terminus. |
| Conjugation | Unconjugated |
| Tag | C-6His |
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Threhalose, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| UniProt ID | P08195 |
| MW | Predicted: 47.89 KDa. Observed: 57-87 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human CD98-His(Cat#orb2995007) at 2μg/ml (100 μl/well)can bind Anti-Human CD98 mAb(Cat#Norb3002386).The ED50 of Anti-Human CD98 mAb(Cat#Norb3002386) is 6.50 ng/ml.. BLI: Loaded Anti-Human CD98 mAb-Fc (Cat#Norb3002386) on Protein A Biosensor, can bind Human CD98-His(Cat#orb2995007) with an affinity constant of 1.04 nM as determined in BLI assay. |
| Expression Region | Arg206-Ala630 |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Alternative names | 4F2 Cell-Surface Antigen Heavy Chain; 4F2hc; 4F2 H Read more... |
| Note | For research use only |
Human | |
0.16-10 ng/mL | |
0.057 ng/mL |
Human | |
0.16-10 ng/mL | |
0.063 ng/mL |
Unconjugated | |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 72.7 KDa. Observed: 75-120 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 47.7 KDa. Observed: 58-80 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 72.7 KDa. Observed: 70-100 KDa, reducing conditions |
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