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Description
Research Area
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human CD96 (C-6His) (Cat#orb2994268)at 2 μg/ml (100 μl/well) can bind Human CD96 Antibody. The ED50 of Human CD96 Antibody is 9.185 ng/ml. (Regularly tested).. BLI: Loaded Human PVR-Fc(Cat#orb2994956) on Protein A Biosensor, can bind Human CD96-His(Cat#orb2994268) with an affinity constant of 0.12 uM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | Val22-Met503 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 54.4 KDa. Observed: 120-150 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human CD96 Protein [orb2994037]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 80.1 KDa. Observed: 120 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD96 Protein [orb2993879]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 80.3 KDa. Observed: 110-160 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human T-cell surface protein tactile (CD96), partial (Active) [orb1095864]
Greater than 95% as determined by SDS-PAGE.
56.3 kDa
Mammalian cell
100 μg, 20 μg, 1 mgHuman CD96 Protein, mFc-His tag [orb689389]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 78.9 kDa after removal of the signal peptide. The apparent molecular mass of CD96-mFc-His is approximately 130-180 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg

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