Recombinant Human CD64 Protein

SKU: orb2994349

Description

Recombinant Human High Affinity Immunoglobulin Gamma Fc Receptor I is produced by our Mammalian expression system and the target gene encoding Gln16-Pro288 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human IgG1(Cat#orb2993960) on Protein A Biosensor, can bind Human CD64-His(Cat#orb2994349) with an affinity constant of 0.11 nM as determined in BLI assay. Loaded Anti-Human CD28 mAb(Cat#GMP-A063) on Protein A Biosensor, can bind Human CD64-His(Cat#orb2994349) with an affinity constant of 0.99 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 31.7 KDa. Observed: 50-70 KDa, reducing conditions
• Expression Region: Gln16-Pro288
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD64; FCGR1A

Quick Database Links

UniProt

P12314