Recombinant Human CD45 Protein

SKU: orb2993061

Description

Recombinant Human Receptor-type Tyrosine-protein Phosphatase C is produced by our Mammalian expression system and the target gene encoding Gln26­Lys577 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human CD45 (C-6His)(Cat#orb2993061) at 2μg/ml (100 μl/well) can bind Anti-Human CD45 Antibody(APA_bio, Research Grade)(Cat#orb2992507).The ED50 of Anti-Human CD45 Antibody(APA_bio, Research Grade)(Cat#orb2992507) is 33.35ng/ml.(Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 61.6 KDa. Observed: 110-180 KDa, reducing conditions
• Expression Region: Gln26-­Lys577
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B220; CD45 antigen; CD45; CD45R; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C

Quick Database Links

UniProt

P08575