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Human CD32a (R167)
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD32a (R167)-6His (Cat#orb2995147) with an affinity constant of 0.547 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 21.61 KDa. Observed: 25-32 KDa, reducing conditions |
| Expression Region | Ala36-Ile218 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CD32a (R167) Protein, hFc Tag [orb1173713]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46.5 kDa after removal of the signal peptide.The apparent molecular mass of CD32a(R167)-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μgRecombinant Human Fc gamma RIIA/CD32a (R167), His-Avi (HEK293) [orb1974358]
>90% as determined by SDS-PAGE
24.1 kDa
100 μg, 500 μg, 20 μgBiotinylated Human Fc gamma RIIA / CD32a (R167) Protein, His, (MALS & SPR verified) [orb3123366]
25 μg, 200 μgHuman Fc gamma RIIA / CD32a (R167) Protein, His Tag (MALS & SPR verified) [orb3123373]
50 μg, 1 mg, 100 μg

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Protocol Information
Human CD32a (R167) (orb2995147)
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