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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-CD316 antibody at 2μg/ml (100 μl/well) can bind Recombinant Human CD316 (C-Fc)(Cat#orb2993833)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human CD316 (C-Fc)(Cat#orb2993833) is 0.22 μg/ml.(Regularly tested).. BLI: Loaded Anti-Human CD316 antibody on SA Biosensor, can bind Recombinant Human CD316 (Cat#orb2993833) with an affinity constant of 14.1 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc |
| Expression Region | Arg28-Thr579 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 85.7 KDa. Observed: 90-120 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 8% Trehalose, 50mM NaCl, 4% Mannitol，0.02% Tween80，pH 8.5 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Immunoglobulin Superfamily Member 8; IgSF8; CD81 Partner 3; Glu-Trp-Ile EWI Motif-Containing Protein 2; EWI-2; Keratinocytes-Associated Transmembrane Protein 4; KCT-4; LIR-D1; Prostaglandin Regulatory-Like Protein; PGRL; CD316; IGSF8; CD81P3; EWI2; KCT4
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Predicted: 59.6 KDa. Observed: 70-85 KDa, reducing conditions
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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