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Human CD27L

SKU: orb2992795

Description

Recombinant Human CD27 ligand is produced by our Mammalian expression system and the target gene encoding Gln45-­Pro193 is expressed with a 6His, Flag tag at N-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Recombinant Human CD27L (N-6His-Flag)(Cat#orb2992795) at 2μg/ml (100 μl/well) can bind Human CD70 Antibody (6E9) (Cat#orb2992601). The ED50 of Human CD70 Antibody (6E9) (Cat#orb2992601) is 1.645 ng/ml. (Regularly tested)
TagN-6His-Flag
Molecular WeightPredicted: 21.5 KDa. Observed: 24-36 KDa, reducing conditions
Expression RegionGln45­Pro193
PuritySDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD70 antigen; CD70; CD27 ligand; Tumor necrosis factor ligand superfamily member 7; CD27LG; TNFSF7; CD27L

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Human CD27L (orb2992795)

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