Human CD16a

SKU: orb2994215

Description

Recombinant Human Fc Gamma RIIIA (F176) is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a 6His tag at the C-terminus. It is identical to FCGR3A158F/V in the reference.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD16a (F176)-His (Cat#orb2994215) with an affinity constant of 1.35 uM as determined in BLI assay. (Regularly tested) Loaded Human CD16a (F176)-His(Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human HER2 mAb(Cat#GMP-A062) with an affinity constant of 3.4 uM as determined in BLI assay. Loaded Human CD16a (F176)-His(Cat#orb2994215) on HIS1K Biosensor, can bind Anti-Human CD16 mAb(Cat#GMP-A091) with an affinity constant of 0.07 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 22.7 KDa. Observed: 35-50 KDa, reducing conditions
• Expression Region: Gly17-Gln208
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII;FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3

Quick Database Links

UniProt

P08637