Recombinant Human Cardiotrophin-1

SKU: orb1905945

Description

Recombinant Human Cardiotrophin-1

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0x106 IU/mg.
• Molecular Weight: Approximately 21.2 kDa, a single non-glycosylated polypeptide chain containing 201 amino acids.
• Protein Sequence: MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 0.1 EU/ug of rHuCT-1 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q16619