Human APRIL

• Catalog Number: orb2994084
• Category: Proteins
• Description: Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus.
• Conjugation: Unconjugated
• Tag: N-Flag-His
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 1M NaCl, pH 8.3.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: O75888
• MW: Predicted: 50 KDa. Observed: 60 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human APRIL -Flag-His(Cat#orb2994084) at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc(Cat#orb2994273).The ED50 of Cynomolgus BCMA-Fc(Cat#orb2994273) is 4.23 ng/ml.. BLI: Loaded Cynomolgus BCMA-Fc(Cat#orb2994273) on Protein A Biosensor, can bind Human APRIL-His(Cat#orb2994084) with an affinity constant of 0.005 nM as determined in BLI assay.
• Expression Region: Lys112-Leu250
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Tumor necrosis factor ligand superfamily member 13
• Note: For research use only