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Recombinant Human ALCAM Protein

SKU: orb2992872

Description

Recombinant Human CD166 antigen is produced by our Mammalian expression system and the target gene encoding Trp28-Ala526 is expressed with a 6His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityBLI: Loaded Recombinant Mouse CD6 (C-6His) (Cat#orb2994104) on SA Biosensor, can bind Recombinant Human ALCAM (C-6His) (Cat#orb2992872) with an affinity constant of 0.222 μM as determined in BLI assay. (Regularly tested)
TagC-6His
Molecular WeightPredicted: 56.8 KDa. Observed: 68-115 KDa, reducing conditions
Expression RegionTrp28-Ala526
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD166 antigen; Activated leukocyte cell adhesion molecule; CD166; ALCAM; MEMD

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Recombinant Human ALCAM Protein (orb2992872)

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