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E.coli Trp A
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | E.coli |
| Tag | Tag free |
| Molecular Weight | Predicted: 28.7 KDa. Observed: 27 KDa, reducing conditions |
| Expression Region | Met1-Ser268 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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−E.coli Trp synthase [orb2993218]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 28.7&43.8 KDa. Observed: 28&40-50 KDa, reducing conditions
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 43.8 KDa. Observed: 38-48 KDa, reducing conditions
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Protocol Information
E.coli Trp A (orb2993222)
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