Recombinant Cynomolgus PD-L1 Protein

SKU: orb2994783

Description

Recombinant Cynomolgus CD274 Molecule is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Cynomolgus
• Biological Activity: ELISA: Immobilized Cynomolgus PD-L1-Fc(Cat#orb2994783) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb(Cat#orb2993144).The ED50 of Anti-Human PDL1 mAb(Cat#orb2993144) is 8.15 ng/ml.. BLI: Loaded Biotinylated Human PD-1-His-Avi(Cat#orb2993936) on SA Biosensor, can bind Cynomolgus PD-L1-Fc (Cat#orb2994783) with an affinity constant of 14.1 nM as determined in BLI assay.
• Tag: C-Fc
• Molecular Weight: Predicted: 52.4 KDa. Observed: 73 KDa, reducing conditions
• Expression Region: Phe19-Thr239
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Quick Database Links

UniProt

G7PSE7