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Cynomolgus GLP1R N-ECD

SKU: orb2992903

Description

Recombinant Cynomolgus Glucagon-like peptide 1 receptor is produced by our Mammalian expression system and the target gene encoding Arg24-Glu139 is expressed with a His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginCynomolgus
Biological ActivityELISA: Immobilized Recombinant GLP1R N-ECD (C-6His) (Cat#orb2992903) at 5 μg/ml (100 μl/well) can bind Anti-GLP1R antibody (Human IgG1). The ED50 of Anti-GLP1R Antibody (Human IgG1) is 32.82 ng/ml. (Regularly tested).. BLI: Loaded Recombinant Cynomolgus GLP1R N-ECD (C-6His) (Cat#orb2992903) on HIS1K Biosensor, can bind Anti-GLP-1R Antibody (Human IgG1) with an affinity constant of 2.45 pM as determined in BLI assay. (Regularly tested)
TagC-6His
Molecular WeightPredicted: 14.4 KDa. Observed: 26-40 KDa
Expression RegionArg24-Glu139
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
DisclaimerFor research use only

Alternative Names

Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R; GLP1R
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Cynomolgus GLP1R N-ECD (orb2992903)

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