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| Catalog Number | orb2992903 |
|---|---|
| Category | Proteins |
| Description | Recombinant Cynomolgus Glucagon-like peptide 1 receptor is produced by our Mammalian expression system and the target gene encoding Arg24-Glu139 is expressed with a His tag at C-terminus. |
| Tag | C-6His |
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| UniProt ID | F8V479 |
| MW | Predicted: 14.4 KDa. Observed: 26-40 KDa |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Cynomolgus |
| Biological Activity | ELISA: Immobilized Recombinant GLP1R N-ECD (C-6His) (Cat#orb2992903) at 5 μg/ml (100 μl/well) can bind Anti-GLP1R antibody (Human IgG1). The ED50 of Anti-GLP1R Antibody (Human IgG1) is 32.82 ng/ml. (Regularly tested).. BLI: Loaded Recombinant Cynomolgus GLP1R N-ECD (C-6His) (Cat#orb2992903) on HIS1K Biosensor, can bind Anti-GLP-1R Antibody (Human IgG1) with an affinity constant of 2.45 pM as determined in BLI assay. (Regularly tested) |
| Expression Region | Arg24-Glu139 |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months. |
| Alternative names | Glucagon-like peptide 1 receptor; GLP-1 receptor; Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
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