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Recombinant Cynomolgus CDCP1 Protein

SKU: orb2992960

Description

Recombinant Cynomolgus monkey CUB domain-containing protein 1 is produced by ou rMammalian expression system and the target gene encoding Phe30-Thr667 is expressed with a 6His tag at C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginCynomolgus monkey
Biological ActivityELISA: Immobilized Anti-CDCP1-NTF antibody(10D7) at 2μg/ml (100 μl/well) can bind Recombinant Cynomolgus CDCP1 (C-6His)(Cat#orb2992960). The ED50 of Recombinant Cynomolgus CDCP1 (C-6His)(Cat#orb2992960) is 10.55 ng/ml. (Regularly tested). BLI: Loaded Recombinant Cynomolgus CDCP1 (C-6His)(Cat#orb2992960) on HIS1K Biosensor, can bind Anti-CDCP1-NTF antibody(10D7) with an affinity constant of 50.4 pM as determined in BLI assay. (Regularly tested)
TagC-6His
Expression RegionPhe30-Thr667
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated
MWPredicted: 72.8 KDa. Observed: 92-135 KDa, reducing conditions
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CUB domain-containing protein 1; CDCP1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein (SIMA135); CD318; TRASK

Similar Products

  • Cynomolgus CDCP1 Protein, His Tag [orb2321239]

    Unconjugated

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 73.4 kDa after removal of the signal peptide. The apparent molecular mass of cCDCP1-His is approximately 100-130 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg
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Protein Handling and Storage Guide
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Recombinant Cynomolgus CDCP1 Protein (orb2992960)

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