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Cynomolgus BTN3A1
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Cynomolgus |
| Biological Activity | ELISA: Immobilized Recombinant Cynomolgus BTN3A1 (C-6His)(Cat#orb2992863) at 5 μg/ml (100 μl/well) can bind Anti-BTN3A mAb (Human IgG1). The ED50 of Anti-BTN3A mAb (Human IgG1) is 3.0 ng/ml. (Regularly tested). BLI: Loaded Recombinant Cynomolgus BTN3A1 (C-6His) (Cat#orb2992863) on HIS1K Biosensor, can bind Anti-BTN3A antibody (Human IgG1) with an affinity constant of 1.58 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 24.3 KDa. Observed: 24-36 KDa, reducing conditions |
| Expression Region | Gln1-Ser218 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Cynomolgus BTN3A1 Protein, His Tag [orb1291773]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 24.3 kDa after removal of the signal peptide. The apparent molecular mass of cBTN3A1-His is approximately 25-35 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg

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Cynomolgus BTN3A1 (orb2992863)
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