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Cynomolgus BTN3A1

SKU: orb2992863

Description

Recombinant Cynomolgus Butyrophilin subfamily 3 member A1 is produced by our Mammalian expression system and the target gene encoding Gln1-Ser218 is expressed with a 6His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginCynomolgus
Biological ActivityELISA: Immobilized Recombinant Cynomolgus BTN3A1 (C-6His)(Cat#orb2992863) at 5 μg/ml (100 μl/well) can bind Anti-BTN3A mAb (Human IgG1). The ED50 of Anti-BTN3A mAb (Human IgG1) is 3.0 ng/ml. (Regularly tested). BLI: Loaded Recombinant Cynomolgus BTN3A1 (C-6His) (Cat#orb2992863) on HIS1K Biosensor, can bind Anti-BTN3A antibody (Human IgG1) with an affinity constant of 1.58 nM as determined in BLI assay. (Regularly tested)
TagC-6His
Molecular WeightPredicted: 24.3 KDa. Observed: 24-36 KDa, reducing conditions
Expression RegionGln1-Ser218
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Butyrophilin subfamily 3 member A1; CD277; BTN3A1; BTF5

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  • Cynomolgus BTN3A1 Protein, His Tag [orb1291773]

    Unconjugated

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 24.3 kDa after removal of the signal peptide. The apparent molecular mass of cBTN3A1-His is approximately 25-35 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg
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Cynomolgus BTN3A1 (orb2992863)

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