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Description
Research Area
Immunology & Inflammation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg. |
| Molecular Weight | Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids. |
| Protein Sequence | QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rCaIFN-γ as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 25 mM Sodium Succinate, pH 5.0, 60 mM NaCl, with 0.1 % Tween-80. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Canine IFNG protein [orb633336]
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Greater than 95% by SDS-PAGE gel analyses
16.8 KDa
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Recombinant Canine Interferon-gamma (orb1906143)
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