Recombinant Bifunctional ligase/repressor BirA, His

SKU: orb1905962

Description

Recombinant Bifunctional ligase/repressor BirA, His

Research Area

Molecular Biology

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Measured by its ability to generate pyrophosphate from the biotinylation reaction. The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (ryPPA1). The specific activity is > 10.0 pmol/min/μg, as measured under the described conditions.
• Molecular Weight: Approximately 36.4 kDa, a single non-glycosylated polypeptide chain containing 329 amino acids, with 6 x His at the C-terminus.
• Protein Sequence: MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE KLEHHHHHH
• Purity: > 95 % by SDS-PAGE analyses.
• Endotoxins: Less than 0.1 EU/ugof rBirA, His as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
• Form/Appearance: Supplied as a 0.2 μm filtered solution in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only