Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit

• Catalog Number: orb781025
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog(JUN).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: P17325
• Sensitivity: 0.054 ng/mL
• Alternative names: C-Jun ELISA Kit, AP1 ELISA Kit, CJun ELISA Kit, p3
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat JUN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat JUN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat JUN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat JUN in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.