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| Catalog Number | orb780362 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 2, Mitochondrial(UCP2). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 0.16-10 ng/mL |
| Assay Time | 3.5h |
| Concentration | 10 ng/mL |
| UniProt ID | P56500 |
| Sensitivity | 0.058 ng/mL |
| Alternative names | SLC25A8 ELISA Kit, UCPH ELISA Kit, Solute carrier Read more... |
| Note | For research use only |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UCP2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
