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| Catalog Number | orb2667582 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Troponin I Type 2, Fast Skeletal (TNNI2) ELISA Kit |
| UniProt ID | P27768 |
| Application notes | standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNNI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNNI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNNI2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 78.13-5000 pg/mL |
| Sensitivity | 23.3 pg/mL |
| Sample Types | Serum, plasma and other biological fluids. |
| Alternative names | AMCD2B; DA2B; FSSV; Troponin I Fast Twitch 2; Trop Read more... |
| Note | For research use only |
