Rat Transthyretin (TTR) ELISA Kit

SKU: orb2667700

Description

Rat Transthyretin (TTR) ELISA Kit

Images & Validation

• Application Notes:
  standard: 500 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TTR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TTR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TTR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TTR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 µg/mL
• Sensitivity: 2.1 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TBPA; PA; PLB; ATTR; PALB; Prealbumin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

Quick Database Links

UniProt

P02767