Rat Transferrin Receptor (TFR) ELISA Kit

SKU: orb2667176

Description

Rat Transferrin Receptor (TFR) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Application Notes:
  standard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 µg/mL
• Sensitivity: 0.053 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

Quick Database Links

UniProt

Q99376

References

Jia-Kui Sun 1, Xiao-Tian Huangfu 1, Xiang Yin 1, Shuai Nie 1, Yi-Hang Deng 1, Zi-Yu Ye 1, Xue-Hui Zhou 1, Xiang Wang 1, Yan-Na Si Ferroptosis is involved in the IL-9-induced intestinal barrier injury in sepsis: an experimental animal and translational study Int J Surg, (2025)

PubMed: 40391960

Applications

ELISA

Reactivity

Rat