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Rat Tight Junction Protein 1 (TJP1) ELISA Kit

Catalog Number: orb781039

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb781039
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1(TJP1).
Tested applicationsELISA
ReactivityRat
Range0.16-10 ng/mL
Assay Time3.5h
Concentration10 ng/mL
UniProt IDA0A0G2K2P5
Sensitivity0.054 ng/mL
Alternative namesZO1 ELISA Kit, Zona Occludens 1 ELISA Kit, Zona oc
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TJP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TJP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TJP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TJP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates, cell lysates and other biological fluids
Expiration DatePlease enquire.
Rat Tight Junction Protein 1 (TJP1) ELISA Kit

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