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| Catalog Number | orb776471 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH). |
| Reactivity | Rat |
| Concentration | 500 pg/mL |
| UniProt ID | P01150 |
| Tested applications | ELISA |
| Application notes | standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 7.82-500 pg/mL |
| Sensitivity | 2.52 pg/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | TRF ELISA Kit, Thyrotropin-Releasing Factor ELISA Read more... |
| Note | For research use only |
Rat TRH ELISA Kit [orb410782]
Rat
0.5 pg/mL-100 pg/mL
0.25 pg/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T- Item 1 of 1
Rat Thyrotropin Releasing Hormone (TRH) ELISA Kit [orb1806816]
Rat
7.81-500pg/mL
4.69 pg/mL
96 T, 48 T
