Cart summary

You have no items in your shopping cart.

Rat Thyroid Hormone Receptor Alpha (THRa) ELISA Kit

Catalog Number: orb780489

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780489
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Alpha(THRa).
Tested applicationsELISA
ReactivityRat
Range0.63-40 ng/mL
Assay Time3.5h
Concentration40 ng/mL
UniProt IDP63059
Sensitivity0.272 ng/mL
Alternative namesTHR-A ELISA Kit, NR1A1 ELISA Kit, AR7 ELISA Kit, E
Read more...
NoteFor research use only
Application notesstandard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat THRa in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates and other biological fluids.
Expiration DatePlease enquire.
Rat Thyroid Hormone Receptor Alpha (THRa) ELISA Kit