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| Catalog Number | orb2668000 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Thrombospondin-1 (TSP-1) ELISA Kit |
| UniProt ID | Q71SA3 |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 1.56-100 ng/mL |
| Sensitivity | 0.46 ng/mL |
| Sample Types | Plasma |
| Alternative names | TSP1; Thrombospondin-1p180; THBS1; Thrombospondin Read more... |
| Note | For research use only |
Rat TSP-1 ELISA Kit [orb411287]
Rat
6.25 ng/mL-400 ng/mL
1.56 ng/mL
96 T, 10 x 96 T, 5 x 96 T, 48 T, 24 T- Item 1 of 1
- Item 1 of 1
- Item 1 of 1
