Rat Thrombopoietin (TPO) ELISA Kit

SKU: orb1291327

Description

Rat TPO (Thrombopoietin) ELISA Kit

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.5 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

THPO, MGDF, MKCSF, ML, MPLLG, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Stimulating Factor

Quick Database Links

UniProt

P49745