Rat Surfactant Associated Protein A2 (SPA2) ELISA Kit

SKU: orb779347

Description

The Rat Surfactant Associated Protein A2 (SPA2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Surfactant Associated Protein A2 (SPA2) in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.061 ng/mL.

Research Area

Respiratory Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPA2 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.061 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

SFTPA2B ELISA Kit, COLEC5 ELISA Kit, SP2A ELISA Kit, SPA1 beta ELISA Kit, SP-A2 ELISA Kit, SPAII ELISA Kit, Collectin-5 ELISA Kit, Pulmonary Surfactant Protein A2 ELISA Kit, 35 kDa pulmonary surfactant-associated protein ELISA Kit, Alveolar proteinosis protein ELISA Kit