Rat Surfactant Associated Protein A (SPA) ELISA Kit

SKU: orb2667251

Description

Rat Surfactant Associated Protein A (SPA) ELISA Kit

Research Area

Infectious Disease & Virology

Images & Validation

• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.78-50 ng/mL
• Sensitivity: 0.17 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein

Quick Database Links

UniProt

P08427