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Catalog Number | orb775291 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Somatostatin(SST) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Somatostatin(SST). |
Tested applications | ELISA |
Reactivity | Rat |
Range | 7.82-500 pg/mL |
Assay Time | 2.5h |
Concentration | 500 pg/mL |
UniProt ID | P60042 |
Sensitivity | 2.83 pg/mL |
Alternative names | GHIH ELISA Kit, SRIF ELISA Kit, SMST ELISA Kit, GH Read more... |
Note | For research use only |
Application notes | standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SST protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SST in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Rat | |
1.56 pg/mL-100 pg/mL | |
0.39 pg/mL |