Rat Solute Carrier Family 7, Member 11 (SLC7A11) ELISA Kit

• Catalog Number: orb781117
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 7, Member 11(SLC7A11).
• Reactivity: Rat
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SLC7A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SLC7A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SLC7A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SLC7A11 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.54 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Alternative names: CCBR1 ELISA Kit, xCT ELISA Kit, Cystine/Glutamate
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.

References

Fatma S Eltyar 1, Dalia M El-Tanbouly 2, Hala F Zaki 3, Rehab M El-Sayed Crosstalk between ferroptosis and NLRP3, a possible therapeutic target in experimentally-induced rheumatoid arthritis: role of P2Y12R inhibition in modulating P53/SLC7A11/ALOX15 signaling Inflammopharmacology, (2025)

PubMed: 40622464

Applications

ELISA

Reactivity

Rat