Rat Soluble Terminal Complement Complex C5b-9 (sC5b-9) ELISA Kit

• Catalog Number: orb2667636
• Category: Assays and Kits
• Description: Rat Soluble Terminal Complement Complex C5b-9 (sC5b-9) ELISA Kit
• Application notes: standard: 2000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 ng/mL
• Sensitivity: 13.1 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: MAC; Membrane Attack Complex; C5b-9; Terminal Comp
• Research Area: Cell Biology, Infectious Disease & Virology, Metab
• Note: For research use only