Rat Sirtuin 6 (SIRT6) ELISA Kit

• Catalog Number: orb782247
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 6(SIRT6).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• Sensitivity: 0.057 ng/mL
• Alternative names: SIR2L6 ELISA Kit, Silent Mating Type Information R
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SIRT6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SIRT6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SIRT6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SIRT6 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.