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Rat Selenoprotein P1, Plasma (SEPP1) ELISA Kit

Rat Selenoprotein P1, Plasma (SEPP1) ELISA Kit

Catalog Number: orb2667517

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667517
CategoryAssays and Kits
DescriptionRat Selenoprotein P1, Plasma (SEPP1) ELISA Kit
Range0.16-10 µg/mL
Assay Time3.5h
UniProt IDP25236
Sensitivity0.052 µg/mL
Alternative namesSEPP; SeP; SEP-P1; SELP
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NoteFor research use only
Application notesstandard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEPP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SEPP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma and other biological fluids.
Expiration DatePlease enquire.