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| Catalog Number | orb776414 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Leukocyte(SELL). |
| Reactivity | Rat |
| Concentration | 10 ng/mL |
| UniProt ID | P30836 |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SELL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SELL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SELL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SELL in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.061 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | CD62L ELISA Kit, L-Selectin ELISA Kit, SEL-L ELISA Read more... |
| Note | For research use only |
Rat L-Selectin ELISA Kit [orb340139]
Rat
78.125 pg/mL-5000 pg/mL
64 pg/mL
10 x 96 T, 5 x 96 T, 48 T, 24 T, 96 T
