Rat S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit

SKU: orb2998753

Description

Rat S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit

Research Area

Cancer Biology, Infectious Disease & Virology, Neuroscience

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100B in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.55 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

S100-B; NEF; S100Beta; S-100 protein subunit beta; S100 protein beta chain; Soluble protein-100B; Soluble protein 100B

Quick Database Links

UniProt

P04631