Rat S100A4(S100 Calcium Binding Protein A4) Microsample ELISA Kit

SKU: orb2997547

Description

Rat S100A4(S100 Calcium Binding Protein A4) Microsample ELISA Kit

Research Area

Cancer Biology, Infectious Disease & Virology

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.06 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

S100-A4; MTS1; 18A2; 42A; CAPL; P9KA; PEL98; Calcium protein; Calvasculin; Metastasin; Protein Mts1; Placental calcium-binding protein; Murine placental homolog

Quick Database Links

UniProt

P05942