Rat RGS6(Regulator Of G Protein Signaling 6) Microsample ELISA Kit

• Catalog Number: orb2997776
• Category: Assays and Kits
• Description: Rat RGS6(Regulator Of G Protein Signaling 6) Microsample ELISA Kit
• UniProt ID: P49801
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RGS6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RGS6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RGS6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RGS6 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.109 ng/mL
• Sample Types: Tissue homogenates and other biological fluids.
• Alternative names: GAP; S914
• Note: For research use only
• Expiration Date: Please enquire.