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Rat Reticulon 4 (RTN4) ELISA Kit
Catalog Number: orb782219
| Catalog Number | orb782219 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reticulon 4(RTN4). |
| Reactivity | Rat |
| Concentration | 10 ng/mL |
| UniProt ID | Q9JK11 |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RTN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RTN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RTN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RTN4 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.06 ng/mL |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids |
| Alternative names | NSP ELISA Kit, ASY ELISA Kit, NOGO ELISA Kit, NSP- Read more... |
| Research Area | Neuroscience |
| Note | For research use only |
| Expiration Date | Please enquire. |
Human RTN4 ELISA Kit [orb405226]
Human
46.88 pg/mL-3000 pg/mL
11.75 pg/mL
48 T, 10 x 96 T, 5 x 96 T, 96 T, 24 T- Item 1 of 1
