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Rat Relaxin (RLN) ELISA Kit

Catalog Number: orb781072

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb781072
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Relaxin(RLN).
Tested applicationsELISA
ReactivityRat
Range31.25-2000 pg/mL
Assay Time3.5h
Concentration2000 pg/mL
Sensitivity8.85 pg/mL
Alternative namesRLXH1 ELISA Kit, Relaxin H1 ELISA Kit, Prorelaxin
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NoteFor research use only
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RLN in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma and other biological fluids
Expiration DatePlease enquire.
Rat Relaxin (RLN) ELISA Kit

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