Rat Regulated On Activation, Normal T-Cell Expressed and Secreted (RANTES) ELISA Kit

SKU: orb1806900

Description

Rat Regulated On Activation, Normal T-Cell Expressed and Secreted (RANTES) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RANTES. You can calculate the concentration of Rat RANTES in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCL5, SCYA5, SISd, TCP228, D17S136E

Quick Database Links

Gene Symbol

RANTES

UniProt

P50231