Rat Pyruvate Kinase, Muscle (PKM2) ELISA Kit

SKU: orb775379

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle(PKM2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKM2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.94-60 ng/mL
• Sensitivity: 0.42 ng/mL
• Concentration: 60 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

M2PK ELISA Kit, PKM ELISA Kit, M2-PK ELISA Kit, PKM1 ELISA Kit, CTHBP ELISA Kit, OIP3 ELISA Kit, PK3 ELISA Kit, PKM ELISA Kit, TCB ELISA Kit, THBP1 ELISA Kit, Pyruvate kinase muscle isozyme ELISA Kit, Thyroid hormone-binding protein 1 ELISA Kit, Cytosolic thyroid hormone-binding protein ELISA Kit

Quick Database Links

UniProt

P11980