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Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit

Catalog Number: orb779854

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779854
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Beta 1(PKCb1).
ReactivityRat
Concentration10 ng/mL
UniProt IDP68403
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKCb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKCb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKCb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKCb1 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.056 ng/mL
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Alternative namesPKC-B1 ELISA Kit, PRKCB ELISA Kit, PRKCB1 ELISA Ki
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NoteFor research use only
Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit