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| Catalog Number | orb782225 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Alpha(PKBa). |
| Reactivity | Rat |
| Concentration | 10 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKBa in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.06 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | AKT1 ELISA Kit, AKT ELISA Kit, PKB-A ELISA Kit, Pr Read more... |
| Note | For research use only |
