Rat Procollagen III N-Terminal Propeptide (PIIINP) ELISA Kit

SKU: orb2667645

Description

Rat Procollagen III N-Terminal Propeptide (PIIINP) ELISA Kit

Research Area

Gastroenterology & Hepatology, Metabolism Research, Musculoskeletal & Connective Tissue Research

Images & Validation

• Application Notes:
  standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.13 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide