Rat pro interleukin 1 beta (pro-IL1b) Microsample ELISA Kit

• Catalog Number: orb2997486
• Category: Assays and Kits
• Description: Rat pro interleukin 1 beta (pro-IL1b) Microsample ELISA Kit
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 16.7 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Alternative names: pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β
• Note: For research use only
• Expiration Date: Please enquire.