Rat Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) ELISA Kit

SKU: orb781834

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5(KCNJ5).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KCNJ5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KCNJ5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KCNJ5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KCNJ5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Kir3.4 ELISA Kit, CIR ELISA Kit, KATP1 ELISA Kit, GIRK4 ELISA Kit, G protein-activated inward rectifier potassium channel 4 ELISA Kit, Cardiac inward rectifier ELISA Kit, Inward rectifier K(+) channel Kir3.4 ELISA Kit, Heart KATP channel ELISA Kit

Quick Database Links

UniProt

P48548